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Bacteriophage T4 cytosine-containing DNA is cleaved at a single site by the restriction endonuclease, Bam H1. The site lies within the late region of the T4 genome, close to, or within, gene 8, one of the structural genes of the phage particle baseplate.

THE BAM H1 RESTRICTION SITE IN THE BACTERIOPHAGE T4 CHROMOSOME IS LOCATED IN OR NEAR GENE 8