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The journal FEMS Microbiology Letters covers all aspects of microbiology including virology. On which scientific shoulders do the papers published in this journal stand? Which are the classic papers used by the authors? We aim to answer these questions in this study by applying the Reference Publication Year Spectroscopy (RPYS) analysis to all papers published in this journal between 1977 and 2017. In total, 16 837 publications with 410 586 cited references are analyzed. Mainly, the studies published in the journal FEMS Microbiology Letters draw knowledge from methods developed to quantify or characterize biochemical substances such as proteins, nucleic acids, lipids, or carbohydrates and from improvements of techniques suitable for studies of bacterial genetics. The techniques frequently used for studying the genetic of microorganisms in FEMS Microbiology Letters' studies were developed using samples prepared from microorganisms. Methods required for the investigation of proteins, carbohydrates, or lipids were mostly transferred from other fields of life science to microbiology.

Influential cited references in FEMS Microbiology Letters: lessons from Reference Publication Year Spectroscopy (RPYS)