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colonization isolates was achieved by API20E and ID32GN and by Vitek2-system in clinical isolates. ESBL-producers were confirmed by E-test and the double-disk-synergy-test. PCR was performed for detection of TEM/OXA/SHV/CTX-Mgroup1/CTX-M-15 genes, phylogenetic-groups, O25b-ST131clonal-group, genes coding for resistance to non-lactamantibiotics and twenty-two virulence-factors. Sequencing was performed using group-specific primers for -lactamase-genes. E. coli isolates relationship was addressed by pulsed-field-gelelectrophoresis (PFGE). Results: Fifty-eight CTX-M-15-producing-E. coli-O25b-ST131 isolates were detected and clustered in nine PFGE-profiles ( 80%homology). Each PFGE type contains clinical isolates from different products, and intestinal-colonization isolates. These isolates showed also extended-resistance-phenotype to nonlactam-antibiotics and a virulent profile. Conclusions: Clonality relationships of CTX-M-producing-E. coli-O25bST131, alert to the circulation of patients between different types of care, favoring the dissemination of these multiresistant bacteria in elder-care network. Intestinal colonization is a silent form of dissemination of CTX-M-15-producing-E. coli and resistance genes, promoted by patient circulation through the healthcare network.

Evaluation of sound pressure levels of infrassons and low frequency noise in Coentral Grande