English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background Intestinal dysbiosis has been consistently described in patients with inflammatory bowel disease (IBD), particularly about changes in gut microbiota’s composition and reduction of biodiversity. The identification of a characteristic microbiota could bring to a better understanding of ulcerative colitis (UC) pathogenesis and to find possible microbial markers of disease activity in UC. The primary purpose of our study has been to evaluate the different compositions of gut microbiota between subjects with UC and healthy controls and between patients in remission and patients with severe colitis. Methods A total of 64 subjects were enrolled: 18 with severe colitis (sUC), 22 UC patients in remission (rUC) and 24 healthy controls (HC). Faecal samples were analysed using the Illumina technique (16S NGS). Statistical analyses were performed with Kruskal–Wallis test. Shannon index and equipartition index were calculated to evaluate α-diversity between the samples and then were compared trough Student’s t-test. Results Significant statistical differences in relative frequencies of phyla exist between UC patients and HC: frequencies of the phyla Firmicutes (with a median of 48.10% in UC and of 34.31% in HC, p &amp;lt; 0.001) and Actinobacteria (0.67% in UC and 0.17% in HC, p = 0.006) were higher in UC, while frequencies of the phyla Bacteroidetes (48.82% in UC and 54.97% in HC, p = 0.005), Cyanobacteria (0% in UC and 1.28 in HC, p &amp;lt; 0.001) and Verrucomicrobia (0% in UC and 2.58% in HC, p &amp;lt; 0.001) were lower in UC. Significant statistical differences in relative frequencies of phyla were found between patients with rUC and sUC: Fusobacteria (median 0.005% for sUC, 0% for rU, p = 0.05) and Proteobacteria (7.88% in sUC, 3.22% for rUC, p = 0.015) were higher in patients with sUC. There was no difference between the relative frequencies of the other phyla (Firmicutes, Bacteroidetes, Actinobacteria, Cyanobacteria and Verruccomicrobia). The gut microbiota biodiversity has been evaluated through Shannon index and equipartition index. Biodiversity was increased in UC patients respect to HC, while it was similar between rUC and sUC patients. Conclusion The difference between the gut microbiota of rUC and sUC patients is mainly the relative frequency of the phylum Proteobacteria, increased in sUC patients. The α-diversity is similar in these two groups, suggesting a limited role in the influence of the course of the disease.

P843 Intestinal microbiota changes according to disease activity in patients with ulcerative colitis