English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background Most studies investigated the intestinal microbiota using stool instead of mucosa-associated microbiota (MAM). We previously analysed MAM using brushing samples during colonoscopy and reported that Actinobacteria was more abundant and Bacteroidetes was less in the brush samples than those in the faecal samples (J Clin Biochem Nutr. 2019;65:132–137). There are few clinical studies investigating correlations between MAM and serum cytokines. To investigate the diversity and characteristics of MAM in Crohn’s disease (CD) and correlations between MAM and serum levels of inflammatory cytokines. Methods CD patients and non-IBD controls who were planning to undergo routine colonoscopy were recruited. 2L polyethylene glycol (PEG) was used as bowel preparation for colonoscopy. Mucosal brushing samples were taken from the ileum and sigmoid colon. 16S rRNA sequencing using the MiSeq system (Illumina, San Diego, CA, USA) was performed. The V3–V4 regions of the gene encoding 16S rDNA (460 bp) were tailed PCR amplified. Processing of sequence data was analysed using QIIME version 1.8.0. Functional changes in the microbiota were evaluated using PICRUSt. Serum cytokine levels were measured using Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay. Results The participants were 15 CD patients (average age 43 years, 10 men), and 13 non-IBD controls (average age 58 years, 7 men). The CD group showed a decrease in alpha diversity compared with the non-IBD group, and a significant difference was observed between the two groups in the unweighted PCoA analysis. Bifidobacterium (p = 0.04) and Ruminococcus (p = 0.02) were significantly increased, and Roseburia (p = 0.03) and Oscillospira (p = 0.02) were significantly decreased in the CD group. The component of Bifidobacterium positively correlated with serum IL-15 levels. Conclusion MAM in CD group were different from those in non-IBD group, suggesting that certain bacteria may control mucosal immunity.

P839 Analysis of mucosa-associated microbiota and serum cytokines levels in Crohn’s disease