Open AccessAnalysis of Tag1-Like Elements in Arabidopsis thaliana and Their Distribution in Other Plants
Author(s) -
Prarthana Shankar,
Shu Ito,
Masaomi Kato,
Minami Matsui,
Ritsuko Kodaira,
Nobuaki Hayashida,
Mitsuo Okazaki
Publication year - 2001
Publication title -
dna research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 98
eISSN - 1756-1663
pISSN - 1340-2838
DOI - 10.1093/dnares/8.3.107
Subject(s) - transposable element , biology , arabidopsis , arabidopsis thaliana , genome , polymerase chain reaction , genetics , genomic dna , primer (cosmetics) , ecotype , homology (biology) , dna , gene , chemistry , mutant , organic chemistry
Analysis of genomic DNA of Arabidopsis Columbia (Col.) ecotype using a transposon Tag1-specific primer showed the presence of Tag1 homologues which was confirmed by Southern hybridization with a Tag1 probe. Further analysis showed that the homologue, 0.75 kb in length, had inverted repeats at both ends, 8-bp duplicated sequences at the site at which it is located and about 80% homology with Tag1, and was randomly distributed in the Arabidopsis genome. Based on these results, we concluded that these elements are non-autonomous variants of Tag1 and we termed this element sTag1. Using the polymerase chain reaction fragment hybridization technique, we found the distribution of such homologues in other plant species.