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A newly and rapid supercritical fluid chromatography method for the simultaneous determination of 11 active compounds in  Radix Hedysari  samples has been developed and validated. Optimum separation was achieved on a HSS SB C 18  column with a gradient elution at a flow rate of 1.5 mL/min, back pressure of 11.03 Mpa and diode array detector at 260 nm. The results from the quantitative data showed that contents of these 11 active compounds were different from plant regions. Especially the contents of formononetin in the Minxian county are ~6-fold than in wild  Radix Hedysari.  The chromatographic fingerprint of  Radix Hedysari  was recorded under the same chromatographic condition. Data analytic procedure was performed to differentiate the 25 batches of  Radix Hedysari  samples. Data from chromatographic fingerprint were also analyzed using hierarchical cluster analysis. The results showed that 23 batches of  Radix Hedysari  samples had a high similarity (&gt; 0.90) and overall 25 batches of sample were divided into two clusters. Moreover, according to the comparison contents of active compounds in each  Radix Hedysari  samples, the cultivated location of  Radix Hedysari  was successfully distinguished. This method presented good stability, repeatability and precision and would be a useful and reliable approach for the quality control of  Radix Hedysari . Moreover, all target compounds were quantified by ultra-high performance liquid chromatography–time-of-flight mass spectrometry.

Chromatographic Fingerprint Analysis of Radix Hedysari Using Supercritical Fluid Chromatography Coupled with Diode Array Detector