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Identification of both hydroxycinnamic and chlorogenic acids present in aqueous extracts of walnut leaves ( Juglans regia  L.) were carried out by using, for the first time, standard compounds not commercially available for qualitative identification. In particular, in addition to caffeic, ferulic,  p -coumaric and sinapic acids,  cis  and  trans  mono-caffeoylquinic, dicaffeoylquinic, mono-feruloylquinic and  cis  and  trans  mono- p- coumaroylquinic acid isomers were detected and quantified by Ultra High Pressure Liquid Chromatography and the seasonal variations of these secondary metabolites were investigated.

Aqueous extracts of walnut ( Juglans regia L.) leaves: quantitative analyses of hydroxycinnamic and chlorogenic acids