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An accurate, precise and sensitive ultra-performance liquid chromatography tandem mass spectrometric (UPLC-MS-MS) method was developed for the determination of flavonoids, terpene lactones, together with ginkgolic acids in Ginkgo biloba leaves extract (EGb 761). This is the first report of the simultaneous analysis of major active ingredients and toxic components in EGb 761 using UPLC-MS-MS. This analysis afforded good linearity, precision, repeatability and accuracy. In addition, the content of those major bioactive components in EGb 761 prepared by different manufacturers of China was determined to establish the effectiveness of the method. The results indicated that the quantification analysis could be readily utilized as a quality control method for EGb 761 and its other related products using flavonoids, terpene lactones and ginkgolic acids as markers.

journal of chromatographic science

Rapid and Sensitive Determination of Major Active Ingredients and Toxic Components in<i>Ginkgo</i><i>Biloba</i>Leaves Extract (EGb 761) by a Validated UPLC–MS-MS Method

Rapid and Sensitive Determination of Major Active Ingredients and Toxic Components inGinkgoBilobaLeaves Extract (EGb 761) by a Validated UPLC–MS-MS Method