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Two fern species  Asplenium adiantum-nigrum  L. and  Asplenium trichomanes  L. collected from the Kroumiria region (Northwest of Tunisia) were individually submitted to hydrodistillation in a Clevenger type apparatus. Volatile organic compounds were identified by GC–MS and GC–FID. Thus, 35 compounds were identified in  A. adiantum-nigrum  essential oil accounting for 77.5% of the whole constituents dominated by palmitic acid (34.5%); however, only 29 volatiles were identified in  A. trichomanes  showing a high amount of phytol, an odorous diterpene alcohol, representing 14.4% of the total oil contents. The total phenolic content and the antioxidant effects of crude extracts from both pteridophytes were determined using Folin–Ciocalteu and 2,2′-diphenyl-1-picrylhydrazyl free radical-scavenging assays, respectively.  A. adiantum-nigrum  ethyl acetate extract is shown to be lower in total phenolic contents (49.3 mg gallic acid equivalent/g) than similar extract from  A. trichomanes  (55.4 mg GAE/g).

Essential Oil Constituents and Antioxidant Activity of Asplenium Ferns