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N -Acetylcysteine (NAC) is the  N -acetyl derivative of the amino acid  l -cysteine and is extensively used as a medicine to treat a variety of diseases. High-throughput ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS) method has been developed for the quantitative assessment of  N -acetyl- l -cysteine. The method was further applied to study the distribution of the intraperitoneal injected drug into different tissues and plasma of Wistar rats, including liver, kidney, heart, lungs and spleen. The drug was having highest concentration in plasma and liver followed by kidney, lungs, heart and spleen. Method validation studies suggested being linear in the range of 1–15 µg mL −1  for liver, kidney, heart, lungs and spleen and 1–120 µg mL −1  for the plasma. The limit of detection and limit of quantitation were found to be 0.20 and 0.66 µg mL −1 , respectively. The recovery studies suggested that in all the cases, the obtained recovery was in the range of 98.51–101.88%. Our analyses provide a validated UPLC-MS method for the determination of NAC and its successful application for the analysis in plasma and tissues obtained from Wistar rats.

High-Throughput UPLC–MS Method for the Determination of N -Acetyl- l -Cysteine: Application in Tissue Distribution Study in Wistar Rats