English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

A simple, precise, rapid and accurate isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the simultaneous determination of biphenyl (aucuparin and noraucuparin) and dibenzofuran (eriobofuran) phytoalexin from elicitor treated cell culture of  Sorbus aucuparia  (mountain ash). These phytoalexins play crucial role in combating scab disease in many commercially important rosaceous plants, such as apple, pear and mountain ash. The isocratic separation was performed in a Luna C 18  reversed-phase column (250 × 4.6 mm, 5 μm particle size) using a mobile phase of 1 mM trifluoroacetic acid (TFA) in water with methanol [40:60 (v/v)]. Quantization of phytoalexin was carried out on Shimadzu-HPLC system using a Photo Diode Array (PDA) detector at 254 nm by comparing the peak area. Peak purity and identity were confirmed by UV spectroscopy and ESI–MS-MS in the negative ion mode. The different analytical performance parameters such as linearity, accuracy, precision, limit of detection and limit of quantification were determined according to the International Conference on Harmonization guidelines. Linearity was observed in the concentration range of 3–400 µg/mL with excellent correlation coefficient ( R  2  ≥ 0.995). This newly developed method is rapid, easy, cost-effective and can be used for monitoring scab-resistance potential of rosaceous plants.

Development and Validation of a New HPLC Method for the Determination of Biphenyl and Dibenzofuran Phytoalexins in Rosaceae