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A simple, rapid and robust liquid chromatography–tandem mass spectrometry was established and validated for simultaneous quantifications of three pyranocoumarins (praeruptorin A–C) in rat plasma. Following a single-step liquid–liquid extraction, the analytes were separated on a reversed-phase C 18  column with a mobile phase consisting of methanol and 10 mM ammonium acetate solution (70 : 30, v/v) at a constant flow rate of 0.3 mL/min. The linear calibration curves were obtained over the concentration ranges 2.93–1470 ng/mL for praeruptorin A, 1.47–734 ng/mL for praeruptorin B and 2.00–1000 ng/mL for praeruptorin C. The within-batch accuracy was −8.6 to 7.5% for praeruptorin A, −9.5 to 12.0% for praeruptorin B and −10.5 to 12.5% for praeruptorin C, respectively. The between-batch accuracy was −3.5 to 1.4% for praeruptorin A, −8.7 to 3.4% for praeruptorin B and −6.0 to 4.3% for praeruptorin C, respectively. The within-batch and between-batch precisions were ≤13.1 and ≤8.2%, respectively. This method is suitable to simultaneously determine the three pyranocoumarins in plasma and thus to investigate the pharmacokinetics of the pyranocoumarins of  Peucedanum praeruptorum  in rats.

journal of chromatographic science

Simultaneous Quantification of Three Pyranocoumarins of  Peucedanum Praeruptorum  in Rat Plasma by Liquid Chromatography–Tandem Mass Spectrometry: Application to Pharmacokinetic Study