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Five previously unknown fumonisin mycotoxins (iso-FP 1 , iso-FP 2,3a , iso-FP 2,3b , FP 4  and iso-FP 4 ) and three previously described FP analogues (FP 1–3 ) were detected in a solid rice culture inoculated with  Fusarium verticillioides . The fumonisins were characterized by high-performance liquid chromatography–electrospray ionization time-of-flight mass spectrometry (HPLC–ESI-TOFMS) and ion trap mass spectrometry (ITMS). The FP isomers were separated by using a flat gradient on a special, high-coverage C18, narrow-bore HPLC column (YMC-Pack J'sphere ODS H80), which was suggested for the separation of structural isomers. The verified structures of the FP 1–3  mycotoxins, the relative retention times (by HPLC–ESI-TOFMS and ITMS), the exact masses of the molecular ions (by TOFMS) and the masses of the product ions, including the hydrocarbon backbones (by ITMS) of the new compounds, strongly suggested their structures.

journal of chromatographic science

Detection of Previously Unknown Fumonisin P Analogue Mycotoxins in a  Fusarium verticillioides  Culture by High-Performance Liquid Chromatography–Electrospray Ionization Time-of-Flight and Ion Trap Mass Spectrometry