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Palmul-tang, a traditional herbal medicine, is composed of eight herbs ( Ginseng radix ,  Glycyrrhizae radix ,  Hoelen ,  Atractylodis rhizoma ,  Angelicae gigantis radix ,  Cnidii rhizoma ,  Paeoniae radix  and  Rehmanniae radix ) and exhibits various bioactivities, including antiallergic and antitumor effects. In this study, an effective, reliable and accurate high-performance liquid chromatography method has been developed for the simultaneous determination of 11 marker components in Palmul-tang: hydroxymethylfurfural, albiflorin, paeoniflorin, ferulic acid, nodakenin, ginsenoside Rg1, decursinol, glycyrrhizin, 6-gingerol, ginsenoside Rg3 and decursin. All calibration curves of the 11 components indicated excellent linearity (correlation coefficient &gt; 0.9997) within the test range. The limits of detection and quantification of each component were in the ranges of 0.08–1.03 and 0.23–3.11 µg/mL, respectively. The intra-day and inter-day relative standard deviation values were within 1.65 and 2.71%, respectively. The mean recovery values were 94.49 to 101.10%. The established method was successfully applied to the simultaneous determination of 11 major components in 12 commercial samples of Palmul-tang. The developed analytical method is simple and suitable for the quality control of Palmul-tang.

Simultaneous Determination of 11 Major Components in Palmul-tang by HPLC–DAD and LC–MS-MS