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This paper describes tryptophan (TRP) estimation in raw human plasma and rat brain by reversed-phase high-performance liquid chromatography (RP-HPLC). Estimation was carried out on a Purospher STAR C18 column using water–acetonitrile (90:10  v/v , at pH 2.7) mixture at a rate of 1.5 mL/min as mobile phase. Eluents were monitored at 273 nm by an ultraviolet detector. The method was linear (R 2  &gt; 0.999), precise (intra-day and inter-day precision &lt;2%) in the range of 0.25–20 µg/mL. The detection and quantification limits were 0.0144 µg/mL and 0.0437 µg/mL, respectively. In human plasma, Day 1 and Day 2 precision were 0.054–2.29% and 1.66–3.7%; whereas precisions in rat brain were 1.23–2.3% and 0.677–4.2%, respectively. The method was applied to study TRP level in human smokers and in arthritic rat brain. An efficient RP-HPLC method was developed for TRP determination that worked for clinical and research purposes.

Determination of Tryptophan in Raw Materials, Rat Brain and Human Plasma by RP-HPLC Technique