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A simple high-performance liquid chromatographic method with ultraviolet detection is proposed for the estimation of diclofenac potassium and drotaverine hydrochloride in human plasma. Liquid–liquid extraction was carried out with a mixture of dichloromethane–isopropyl alcohol (80:20,  v/v ). Chromatographic separation of the analytes and internal standard was achieved on an analytical 250 × 4.6 mm i.d. reversed-phase Thermo BDS Hypersil C8 (5 µm particle size) column using a mobile phase of acetonitrile–0.02M ammonium acetate buffer (53:47,  v/v ) at pH 3.5. The run time was less than 15 min. Column eluate was monitored at 230 nm. The linearity over the concentration ranges of 25–1500 ng/mL and 32–960 ng/mL was obtained for diclofenac potassium and drotaverine hydrochloride, respectively. The limit of quantification was 25 and 32 ng/mL for diclofenac potassium and drotaverine hydrochloride, respectively. Recoveries of diclofenac potassium and drotaverine hydrochloride from plasma were 97.45% and 98.27%, respectively.

journal of chromatographic science

Simultaneous Determination of Diclofenac Potassium and Drotaverine Hydrochloride in Human Plasma using Reversed-Phase High-Performance Liquid Chromatography