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A heart-cutting two-dimensional gas chromatography (GC) method was developed for the determination of nitrobenzene compounds (NBCs) in mainstream cigarette smoke. For the method, the particulate matter of cigarette smoke was extracted with cyclohexane, purified with a silica solid-phase extraction (SPE) cartridge and analyzed by heart-cutting two-dimensional GC equipped with two electron capture detectors. The heart-cutting two-dimensional GC was achieved by a single-column GC oven equipped with a microfluidic pressure balanced device (Deans switch). Two-dimensional GC was compared to single-dimensional GC and found to be clearly better for the separation of seven NBCs from a complex smoke matrix. The limits of detection ranged from 1.28 to 9.83 ng/mL, spiked recoveries were between 88.3 and 106.8% and relative standard deviation ranged from 2.79 to 12.78%. The NBCs yields of six kinds of Chinese and international cigarettes brands, which were all smoked according to two smoking protocols (International Organization for Standardization and Health Canada Intense smoking regimens), were determined and compared.

Determination of Seven Nitrobenzene Compounds in Mainstream Cigarette Smoke with Heart-Cutting Two-Dimensional Gas Chromatography