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In this study, double-vortex-ultrasonic assisted dispersive liquid-liquid microextraction (DVUDLLME) was applied to determine the concentration of vitamin B9, 5-methyl tetrahydrofolate (5-MeTHF) and vitamin B12 in human serum samples.

journal of chromatographic science

Determination of B Vitamins by Double-Vortex-Ultrasonic Assisted Dispersive Liquid–Liquid Microextraction and Evaluation of their Possible Roles in Susceptibility to COVID−19 Infection: Hybrid Box–Behnken Design and Genetic Algorithm