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The first three statistical moments of the eluted band profiles from reversed-phase liquid chromatographic columns were attempted to be measured and analyzed in this work. And in this first part, the effect of the experimental conditions on the measurement accuracy of the third central moment is investigated. The optimized experimental condition is determined under which the measurement accuracy of the third central moment is much improved. Further analysis of the measurement result of the third central moment (micro(3)) shows that the linear correlation exists between micro(3)u(0)(4) and the flow velocity (u(0)).

journal of chromatographic science

Application of Moment Analysis to Mass Transfer Kinetics of Reversed-Phase Liquid Chromatography: Part 1. Experimental Investigation of Measurement of the Third Central Moment