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An inexpensive, harmless, and quick technique of sample preparation followed by a reversed-phase high-performance liquid chromatography (HPLC) for the simultaneous quantification of trenbolone acetate and 17β-trenbolone in cattle muscle is presented. Analysis by HPLC with photo-diode array detector was performed using a short C1 column with an isocratic mobile phase. The method was validated by the analyses of spiked samples, resulting recoveries (≥ 87.9%; relative standard deviations ≤ 3.4%), analytical total time (&lt; 20 min/sample, where, a batch of 12 samples in 2 h), and quantitation limits (≤ 1.8 ng/g). No harmful organic solvents and reagents were used at all.

A Harmless Method for Determining Trenbolone Acetate Together with 17β-Trenbolone in Beef