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A simple, rapid, and reproducible reverse-phase high-performance liquid chromatographic method is developed for the estimation of boswellic acids, the active constituents in  Boswellia serrata  oleogum resin. The chromatographic separation is performed using a mobile phase consisting of acetonitrile—water (90:10, % v/v) adjusted to pH 4 with glacial acetic acid on a Kromasil 100 C18 analytical column with flow rate of 2.0 mL/min and detection at 260 nm. The elution times are 4.30 and 7.11 min for 11-keto β-boswellic acid (11-KBA) and 3-acetyl 11-keto β-boswellic acid (A-11-KBA), respectively. The calibration curve is linear in the 11.66—58.30 µg/mL and 6.50–32.50 µg/mL range for 11-KBA and A-11-KBA, respectively. The limits of detection are 2.33 µg/mL and 1.30 µg/mL for 11-KBA and A-11-KBA, respectively. The mean recoveries are 98.24% to 104.17% and 94.12% to 105.92% for 11-KBA and A-11-KBA, respectively. The inter- and intra-day variation coefficients are less than 5%. The present method is successfully applied for the estimation of boswellic acids from the market formulations containing  Boswellia serrata  extract.

journal of chromatographic science

A Simple High-Performance Liquid Chromatographic Method for the Estimation of Boswellic Acids from the Market Formulations Containing  Boswellia  serrata Extract