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A simple and specific analytical method for the quantitative determination of five cucurbitane-type triterpenoids isolated from the fruit of  Momordica charantia  is developed. The triterpenoids present in the fruits of  Momordica charantia  are separated with an acetonitrile (0.1% acetic acid)-water (0.1% acetic acid)-methanol (0.1% acetic acid) gradient at a flow rate of 0.5 mL/min. The high-performance liquid chromatography separation was performed on a Phenomenex C18 reversed-phase column. By using an evaporative light scattering detector, the main triterpenoids of  Momordica charantia  could be detected at levels as low as 10 µg/mL. The method was validated for precision, repeatability, and accuracy. The relative standard deviation was between 0.6–4.4%. The method was sensitive, quick, and accurate for the determination of main triterpenes and saponins in  Momordica charantia , and can be used for quality control of  Momordica charantia  and its related dietary supplements.

journal of chromatographic science

Determination and Quantitation of Five Cucurbitane Triterpenoids in  Momordica charantia  by Reversed-Phase High-Performance Liquid Chromatography with Evaporative Light Scattering Detection