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Pueraria lobata  is a rich source of isoflavonoids. The detection and identification of isoflavonoid components from  Pueraria radix  (RP), callus and cell cultures, is very important for the safest and most effective use of kudzu as a medicinal plant, and for the studies on quantitative analysis and secondary metabolism of isoflavonoids  in vitro  cultures. Liquid chromatography is coupled with negative and positive electrospray ionization (ESI) tandem mass spectrometry (MS-MS), and photodiode array detection is used to characterize and detect isoflavonoids in root, callus, and cell samples of  P. lobata . Characteristic product ions of aglycones,  O -glucosides, and  C -glucosides were obtained from the full-scan ESI-MS chromatography of the major peaks and the MS-MS spectra of the protonated ions. Five major components of puerarin, daidzin-6″- O -acetylester, genistin-6″- O -malonylester, biochanin A-7- O -glucoside-6″- O -malonylester, and daidzein are detected and identified from the methanolic extract of  P. lobata  callus cultures. The major isoflavonoid components of  P. lobata  cell suspension cultures are identified as puerarin, daidzin, daidzin-6″- O -acetylester, genistin-6″- O -malonylester, biochanin A-7- O -glucoside- 6″- O -malonylester, genistein-8-C-glucoside-6″- O -malonylester, and daidzein, on the basis of ESI-MS and MS-MS spectra analysis. Likewise, puerarin, daidzin, genistein-6″- O -malonylester, 3′-methoxypuerarin, and daidzein are detected and identified from RP. Of those isoflavonoid components detected, daidzin-6″- O -acetylester is a new isoflavonoid glucoside and is for the first time detected from  P. lobata  cultures  in vitro .

journal of chromatographic science

Identification of Isoflavonoids in Several Kudzu Samples by High-Performance Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry