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The use of gas chromatography (GC)-mass spectrometry (MS), GC-time-of-flight MS (TOFMS), comprehensive two-dimensional GC (GC×GC)-flame ionization detection (FID), and GC×GC-TOFMS is discussed for the characterization of the eight important representative components, including Z-α-santalol,  epi -α-bisabolol, Z-α- trans -bergamotol,  epi -β-santalol, Z-β-santalol,  E , E -farnesol,  Z -nuciferol, and  Z -lanceol, in the oil of west Australian sandalwood ( Santalum spicatum ). Single-column GC-MS lacks the resolving power to separate all of the listed components as pure peaks and allow precise analytical measurement of individual component abundances. With enhanced peak resolution capabilities in GC×GC, these components are sufficiently well resolved to be quantitated using flame ionization detection, following initial characterization of components by using GC×GC-TOFMS.

journal of chromatographic science

Comprehensive Two-Dimensional Gas Chromatography with Flame Ionization and Time-of-Flight Mass Spectrometry Detection: Qualitative and Quantitative Analysis of West Australian Sandalwood Oil