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A simplified determining method for the routine monitoring of residual amprolium in edible chicken tissues (muscle and liver) is developed using a high-performance liquid chromatographic (HPLC) method with a photodiode-array detector after sample cleanup by an Ultrafree-MC/PL centrifugal ultrafiltration unit. For the HPLC determination and identification, a Mightysil RP-4 GP column and a mobile phase of an ethanol-5mM 1-heptanesulfonic acid sodium salt solution (35:65, v/v) using an ion-pairing system with a photodiode-array detector are used. Average recoveries (spiked at 0.3–3.0 µg/g) are &gt; 90%. The inter- and intravariabilities are 1.9–2.4%. The limits of quantitation are 0.22 µg/g for muscle and 0.25 µg/g for liver. The total time and solvent required for the analysis of one sample are &lt; 20 min and &lt; 2 mL of ethanol, respectively. No toxic solvents and regents are used.

Simplified High-Performance Liquid Chromatographic Determination of Residual Amprolium in Edible Chicken Tissues