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Solid-phase microextraction (SPME) coupled with gas chromatography (GC)-ion-trap mass spectrometry (ITMS) is employed to analyze fragrance compounds from different species of eucalyptus trees:  Eucalyptus dunnii, Eucalyptus saligna, Eucalyptus grandis , and hybrids of other species. The analyses are performed using an automated system for preincubation, extraction, injection, and analysis of samples. The autosampler used is a CombiPAL and has much flexibility for the development of SPME methods and accommodates a variety of vial sizes. For automated fragrance analysis the 10- and 20-mL vials are the most appropriate. The chromatographic separation and identification of the analytes are performed with a Varian Saturn 4D GC-ITMS using an HP-5MS capillary column. Several compounds of eucalyptus volatiles are identified, with good reproducibility for both the peak areas and retention times. Equilibrium extraction provides maximal sensitivity but requires additional consideration for the effect of carryover. Preequilibrium extraction allows good sensitivity with minimal carryover.

Automation of Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry Extraction of Eucalyptus Volatiles