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A reversed-phase high-performance liquid chromatographic method is developed for the determination of astragaloside (AGS) IV, which is known as the active constituent of  Radix Astragali . The method uses precolumn derivatization with benzoyl chloride to form the benzoyl ester of AGS IV quantitatively and is carried out with a wide-ranging concentration (0.004–0.080 mg/mL) of the derivatized AGS IV. The eluent consists of 90% methanol, 4% tetrahydrofuran, 6% water, and 0.2% triethylamine, with vitamin D 3  added as the internal standard.

journal of chromatographic science

A Precolumn Derivatization High-Performance Liquid Chromatographic Method with Improved Sensitivity and Specificity for the Determination of Astragaloside IV in  Radix Astragali