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The liquid chromatographic retention mechanism of nitrophenol isomers by isocratic elution is investigated over a range of column temperatures and mobile phase compositions using several β-cyclodextrin (β-CD) bonded stationary phases. The high-performance liquid chromatographic supports are based on silica beads coated with a polymer containing β-CD. Thermodynamic parameters for the solute transfer from the mobile to stationary phases are determined, and complex formation constant values between solute and β-CD are calculated. The thermodynamic data shows that the solute retention mechanism is dependent on the amount of bonded β-CD, and the complexation constant is dependent on temperature and mobile phase composition.

journal of chromatographic science

High-Performance Liquid Chromatography of Structural Isomers with Cyclodextrin-Poly(vinylamine)-Coated Silica Columns, Part III: Retention Mechanism Study of Nitrophenol Derivatives