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This work presents the chromatographic study of the association between 5 polycyclic aromatic hydrocarbons and the aggregates formed by a cationic surfactant in the presence of a high percentage of two different alcohols,  n -propanol (20–50%, v/v) and ethanol (50–75%, v/v). A generalization of the classical equations for micellar liquid chromatography can be used in order to determine the solute-aggregates binding constants. Also, by means of a multiple regression, it is possible to obtain an equation that relates the chromatographic retention (expressed as the inverse of the retention factor) and the surfactant and alcohol concentrations. This equation allows one to carry out an estimate of the association constants for any quantity of alcohol.

journal of chromatographic science

Determination of Solute—Aggregate Binding Constants of Some Polycyclic Aromatic Hydrocarbons by High-Performance Liquid Chromatography in the Presence of CTAB—Alcohol—Water Systems with High Percentages of Alcohol