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This paper describes a method for the determination of honeybee cuticular hydrocarbon and ester composition from a single run using capillary gas chromatography. This method is designed to provide information on individual honeybee profiles with the view to map the quantitative trait loci (QTL) that control their variability. The first part of this work concerns the choice of chromatographic parameters, particularly the comparison of three types of injector: the split/splitless, the on-column, and the recently developed automated solid phase (ASP) injector. The second part refers to the reliability of the results with respect to the above-mentioned objective.

Method Development for the Analysis of Bee Cuticular Hydrocarbons and Esters by Capillary Gas Chromatography