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Determination of chlorinated phenoxyacid herbicides by gas chromatography is complicated by the fact that these compounds exhibit low volatilities due to the hydrogen bonding of their carboxylic acid and phenol functionalities. Furthermore, these functional groups can cause the parent molecule to adsorb on the stationary phase of the column, thus producing asymmetric peaks. Masking of these polar groups can significantly increase the volatility and reduce the adsorption effects of these compounds, yielding products that can undergo analysis by gas chromatography.

Application of the Base-Promoted Esterification Procedure to the Chlorophenoxy Acid Herbicides of U.S. EPA Method 515.3