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This paper describes a new, highly sensitive, rapid method for the determination of low levels of endogenous melatonin, the pineal and retinal hormone, in fish plasma samples. The combination of solid-phase extraction and high-performance liquid chromatography with fluorescence detection significantly improves the separation and increases the sensitivity of the assay. The proposed method may be a useful alternative to previously reported procedures. The assay should be applicable to plasma and tissue samples from other animals, including humans, with only minor modifications.

journal of chromatographic science

Highly Sensitive and Specific Assay of Plasma Melatonin Using High-Performance Liquid Chromatography with Fluorescence Detection Preceded by Solid-Phase Extraction