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A method for the separation and fractionation of the phenolic compounds atranorin, methyl orsenillate, orsenillic acid, and lecanoric acid from the lichen  Parmotrema tinctorum  (Nyl.) Hale ( P. tinctorum ) using thin-layer chromatography (TLC) and column chromatography, respectively, is described. TLC of lichen extract with an acid-free developing solvent on silica gel G containing 1 % oxalic acid is carried out, and the compounds are visualized as yellow spots when sprayed with 10% sulfuric acid in methanol followed by heating at 110°C. For the fractionation of pure lichen compounds, column chromatography is carried out using silica gel impregnated with 1 % oxalic acid. To our knowledge, it is also the first report on the isolation and characterization of methyl orsenillate and orsenillic acid from  P. tinctorum  (Nyl.) Hale.

Improved Chromatographic Method for the Purification of Phenolic Constituents of the Lichen Parmotrema tinctorum (Nyl.) Hale