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The Carbowax-20M liquid phase is becoming the standard in gas chromatography for general fatty acid analyses because it simplifies the work and reduces the chance for errors in identification of fatty acids. Isothermal analysis using the Carbowax-20M column, as opposed to temperature-programming, provides further simplification and ruggedness to the analysis. An extensive compilation of characteristic separation factors for 59 different fatty acid methyl esters has appeared, but it included gas-liquid chromatography elution information on only four of the approximately 20–24 isomeric octadecenoates that occur in foods containing ruminate fats and partially-hydrogenated vegetable oils. This contribution serves to fill that void by providing the equivalent chain length elution factors for 25 additional  trans - and  cis -monoethylenic fatty acid methyl esters.

journal of chromatographic science

Simplifying Fatty Acid Analyses Using a Standard Set of Gas—Liquid Chromatographic Conditions: II. Equivalent Chain Length Values for  cis - and  trans - Isomers of Monoethylenic C 18  Fatty Acid Methyl Esters for Carbowax-20M Liquid Phase