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The separation and determination of several steroidal substances are achieved with capillary supercritical fluid chromatography by using supercritical CO 2  as mobile phase. Different stationary phases are used to compare chromatographic results, optimize the separation, and obtain information about the chromatographic behavior of the substances examined. Unexpected elution orders and problems of poor sensitivity of the flame-ionization detector towards some compounds are discussed. Alternative chromatographic conditions are explored: an electron-capture detector is used with suitably optimized pressure gradients in an attempt to overcome these limitations.

Analysis of Steroids by Capillary Supercritical Fluid Chromatography with Flame-lonization and Electron-Capture Detectors