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A method to simultaneously identify and quantitate the major carboxylic acids, sugars, glycerol, and ethanol in wines and grape musts is proposed. The technique involves isocratic separation, an ion-exchange column, and refractive index and UV detection (at 214 nm) without sample preparation. Statistical evaluation shows that the high-performance liquid chromatographic (HPLC) method is highly reproducible and reliable. The results of the HPLC analyses and the data obtained by standard methods are compared.

Simultaneous Determination of the Major Organic Acids, Sugars, Glycerol, and Ethanol by HPLC in Grape Musts and White Wines