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An ion-pair reversed-phase liquid chromatographic method is used to separate As(III), As(V), arsenobetaïn, arsenocholin, monomethylarsonic, and dimethylarsonic acids in a single run on a common C 18  stationary phase with coupled amperometric and ultraviolet detection. As(III) is not separated from arsenobetam, but only As(III) is detected by amperometry. The calibration curves of the tested species are linear from 0.5 to 3 μmol/L. Coefficient of variation values for intra-assay precision range between 2.5% and 9.2%. The limit of quantitation ranges from 0.1–1 μmol/L, depending on the species concerned.

Arsenic Speciation by Ion-Pair Reversed-Phase Liquid Chromatography with Coupled Amperometric and Ultraviolet Detection