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The liquid chromatographic retention behavior and separation by isocratic elution of aromatic compounds by using several β-cyclodextrin bonded stationary phases is investigated. The high-performance liquid chromatographic supports are based on silica beads coated with a poly(alkylamine) (poly[vinylamine] or PVA). The ability of these supports to separate  ortho, meta , and  para  isomers of some disubstituted benzene derivatives is studied. The contribution of the amino groups of the poly(vinylamine) and of the β-cyclodextrin cavity to the separation process is discussed. Several interactions (inclusion complex formation, acid-base and hydrophobic interactions) are discussed. The effect of the composition of the mobile phase on the separation is also examined.

HPLC of Structural Isomers Using Cyclodextrin-Poly(vinylamine)-Coated Silica Columns, Part II: Retention Behavior and Separation