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We present a meticulous analysis of the heterogeneity in colorectal carcinoma cells based on single cell cloning using our novel miFISH technique allowing simultaneous visualization of numerical aberrations. We show that, despite random chromosomal instability, specific net genomic imbalances are maintained.

carcinogenesis

The evolution of single cell-derived colorectal cancer cell lines is dominated by the continued selection of tumor-specific genomic imbalances, despite random chromosomal instability