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Oxidative stress generated from endogenous and exogenous sources causes oxidative DNA damage. The most frequent mutagenic base lesion 7,8-dihydro-8-oxoguanine and the resulting mismatched adenine are removed by OGG1 and MYH in mammals. Deficiencies in human MYH or mouse MYH and OGG1 result in tumor predisposition but the underlying molecular mechanism is not fully understood. To facilitate the study of the roles of MYH and OGG1 in the protection against oxidative stress, we generated mouse embryonic fibroblast cell lines deficient in these genes.   Myh  and   Ogg1  double knockout cells were more sensitive than wild type to oxidants (hydrogen peroxide and   t  -butyl hydroperoxide), but not to   cis  -platinum or γ-irradiations. The low dosage oxidative stress resulted in more reduction of S phase and increase of G  2  /M phase in    Myh  −/−  Ogg1  −/−   cells than in wild-type cells, but a similar level of cell death in both cells. The oxidants also induced more multinucleated cells in    Myh  −/−  Ogg1  −/−   cells than in wild-type, accompanied by centrosome amplification and multipolar spindle formation. Thus, under oxidative stress,   Myh  and   Ogg1  are likely required for normal cell-cycle progression and nuclear division, suggesting multiple roles of   Myh  and   Ogg1  in the maintenance of genome stability and tumor prevention.

Cells deficient in oxidative DNA damage repair genes Myh and Ogg1 are sensitive to oxidants with increased G 2 /M arrest and multinucleation