English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

N-3 polyunsaturated fatty acids (PUFA) and genistein have been associated with lowered cancer risk by reducing inflammatory prostanoids, cyclooxygenase-2 (COX-2) activity, and altering cell signaling. Few studies have investigated the effect of these compounds in combination on the molecular control of the COX-2 gene. In a series of experiments we examined a potential synchronous action ofn-3 PUFA and genistein in down-regulating COX-2 expression to diminish prostaglandin E2(PGE2) production in MDA-MB-231 human breast cancer cells. Cells were treated with genistein and various PUFA including arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). PGE2concentrations, expression of COX-2, and cell invasiveness were determined. Then-3 PUFA and genistein alone lowered PGE2concentration, and genistein in combination with AA reversed the high level of this prostanoid in cell cultures enriched with AA. The degree of cell invasiveness was reversed by genistein in cell cultures treated with AA and further reduced in those given DHA. Then-3 PUFA, in contrast to AA, reduced COX-2 and NFκB expression. Genistein combined with AA reversed the effects of AA alone on the expression of COX-2 and NFκB. All three fatty acids increased the expression of PPARγ in the cells only when combined with genistein. Our results support the premise that DHA and genistein exert complementary actions whilst genistein is antagonistic to AA for controlling PGE2production as well as invasiveness of MDA-MB-231 cells in culture by modulating the level of NFκB expression.

carcinogenesis

Complementary actions of docosahexaenoic acid and genistein on COX-2, PGE              2               and invasiveness in MDA-MB-231 breast cancer cells