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Motivation : Many approaches have been proposed for the protein identification problem based on tandem mass spectrometry (MS/MS) data. In these experiments, proteins are digested into peptides and the resulting peptide mixture is subjected to mass spectrometry. Some interesting putative peptide features (peaks) are selected from the mass spectra. Following that, the precursor ions undergo fragmentation and are analyzed by MS/MS. The process of identification of peptides from the mass spectra and the constituent proteins in the sample is called protein identification from MS/MS data. There are many two-step protein identification procedures, reviewed in the literature, which first attempt to identify the peptides in a separate process and then use these results to infer the proteins. However, in recent years, there have been attempts to provide a one-step solution to protein identification, which simultaneously identifies the proteins and the peptides in the sample.

Improving protein identification from tandem mass spectrometry data by one-step methods and integrating data from other platforms