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The Basic Local Alignment Search Tool (BLAST) algorithm remains one of the most widely used bioinformatic programs. For many projects, new sequencing technologies and increased database sizes will increase the BLAST output significantly. Frequently, this output is so large that it is no longer able to be processed manually. As BLAST users are increasingly recruited from mainstream biology without any bioinformatic background, user-friendly programs capable of BLAST output visualization, analysis and post-processing are in demand. In this review, freely available BLAST output processing programs are categorized as BLAST output interpreters, BLAST environments, BLAST output parsers or specialized tools. They are evaluated according to their user-friendliness, analysis features and high-throughput data processing capabilities.

BLAST output visualization in the new sequencing era