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The cotyledons of Lupinus angustifolius contain large amounts of cell wall storage polysaccharide (CWSP) composed mainly of (1--&gt;4)-beta-linked D-galactose residues in the form of branches attached to a rhamnogalacturonan core molecule. An exo-(1--&gt;4)-beta-galactanase with a very high specificity towards (1--&gt;4)-beta-linked D-galactan has been isolated from L. angustifolius cotyledons, and shown to vary (activity and specific protein) in step with CWSP mobilization. This work aimed to confirm the hypothesis that galactan is the main polymer retrieved from the wall during mobilization at the ultrastructural level, using the purified exo-galactanase as a probe.

annals of botany

The Role of Exo-(1→4)-β-galactanase in the Mobilization of Polysaccharides from the Cotyledon Cell Walls of Lupinus angustifolius Following Germination