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Using a leaf-disk bioassay, Rohm and Haas experimental ecdysone agonists or Molt Accelerating Compounds (MAC), RH-5992 and RH- 2485, and Penncap-M were evaluated for residue effects on PLR and OBLR neonate larvae. The test was conducted in an apple orchard at the Tree Fruit Research and Extension Center. The trees were 15-yr-old spur type ‘Red Delicious’ on dwarfing roots. The treatments were applied on 4 Aug ai the recommended rate with a handgun sprayer at 300 psi to the point of drip, simulating a dilute spray of approximately 400 gal/acre. Each treatmem was replicated three times with one tree in each. Ten leaves were collected from the interior canopy of each tree at 4, 7, 14, 21 and 28 DAT. Two punches (2.3 cm diameter) were taken from each leaf. Four punches were placed in a petri dish (Falcon 1006, 50 × 9 mm), keeping the leaves from each replication separate. Petri dishes were chosen randomly, and five 1- to 2-d-old leafroller larvae were placed on the leaves. Five petri dishes were prepared for each tree and each leafroller species (75 larvae per treatment). The petri dishes were placed inside a food storage container and kept ai 20°C constant temperature and a photoperiod of 16:8 (L:D) h. Petri dishes were examined after 7 d and larval survival recorded.

Bioassay, Effect of Field-Aged Residue Degradation of Molt Accelerating Compounds and Penncapm, 1994