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Experimental ecdysone agonists or Molt Accelerating Compounds (MAC), RH-5992 and RH-2485, were evaluated using a leaf-dip bioassay to determine their effect on PLR and OBLR larvae. Concentrations of RH-5992 and RH-2485 were diluted in 500 ml of water plus 2 ml of X-77® wetting agent. An untreated control was prepared using water plus the wetting agent. Untreatedi apple leaves were collected from ‘Red Delicious’ trees at the WSU Tree Fruit Research and Extension Center, Wenatchee. Leaves were dipped, then allowed to dry. Two punches (2.3 cm diameter) were taken from each leaf. Four punches were placed in a petri dish (Falcon 1006, 50 × 9 mm). Petri qishes were chosen randomly, and five 1- to 2-d-old leafroller larvae were placed on the leaf disks. The petri dish lid was put in place and dishes were placed inside a food storage container with a moist paper towel to maintain high humidity and kept at 75°F (±2°F) constant temperature and a photoperiod of 16:8 (L:D) h. Petri dishes were examined after 7 d and larval survival recorded. Ten dishes were used for each treatment (50 larvae per treatment).

Bioassay, Effects of Molt Accelerating Compounds (MAC) on Neonate Leafroller Larvae, 1994