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The study was conducted in Lincoln, NE on a Kentucky bluegrass golf course fairway. The turf (100% Kentucky bluegrass) was maintained at a mowing height of 5/s inches and thatch accumulation in the plot area was 0.75 inches (finger compressed, 0.5 inches). Field conditions at the time of application were: soil type, silty clay loam; soil organic matter, 3-5%; soil pH, 6-7; water pH, 7.0; soil moisture, near field capacity Plots were 40 inches × 15 ft and the experimental design was a RCB with 4 replications. Formulations were applied 24 Aug using a Cushman Prototypt Subsurface Applicator configured to place liquid formulations at the soil-thatch interface (average depth, 0.75 inches). Immediately following applications plots were irrigated with 0.5 inches of water. A total of 5.41 inches of rain fell during the post-treatment period. Treatments were evaluated 21 DAT on 14 Sep by removing from each plot three, 8-inch diam turf-soil cores (1.05 ft2 total area) to a depth of 3 inches and counting the number of surviving grubs.

Subsurface Placement of Bioneem and Entomopathogenic Nematodes for Control of Annual White Grubs, 1993