Open AccessTHE SYNERGISTIC ACTION OF ETHANOL AND NERVE GROWTH FACTOR IN THE INDUCTION OF NEURONAL NITRIC OXIDE SYNTHASE
Author(s) -
Yume T. Phung
Publication year - 1999
Publication title -
alcohol and alcoholism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.747
H-Index - 99
eISSN - 1464-3502
pISSN - 0735-0414
DOI - 10.1093/alcalc/34.4.506
Subject(s) - staurosporine , nerve growth factor , nitric oxide synthase , nitric oxide , ethanol , chemistry , endocrinology , medicine , mechanism of action , neuronal nitric oxide synthase , protein kinase c , signal transduction , biochemistry , biology , in vitro , receptor
Ethanol alone had no effect on neuronal nitric oxide synthase (nNOS) expression in PC12 cells. However, in the presence of nerve growth factor (NGF), nNOS expression was amplified (threefold, P < 0.05), compared to NGF alone. This increase was eliminated with pretreatment of PC12 cells with staurosporine, suggesting that the effects of ethanol on nNOS expression are mediated by a protein kinase C-dependent pathway.
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