Open AccessSEMI-AUTOMATED CARBOHYDRATE-DEFICIENT TRANSFERRIN IN PRIMARY BILIARY CIRRHOSIS: A PILOT STUDY
Author(s) -
Pamela Bean,
Asgeir Husa,
Karsten Liegmann,
Erling Sundrehagen
Publication year - 1998
Publication title -
alcohol and alcoholism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.747
H-Index - 99
eISSN - 1464-3502
pISSN - 0735-0414
DOI - 10.1093/alcalc/33.6.657
Subject(s) - carbohydrate deficient transferrin , primary biliary cirrhosis , densitometry , medicine , gastroenterology , transferrin , immunoassay , gold standard (test) , false positive paradox , alcohol , immunology , chemistry , alcohol consumption , antibody , biochemistry , machine learning , computer science
Primary biliary cirrhosis (PBC) is one of the few non-alcohol induced liver pathologies which causes false positive results in the evaluation of carbohydrate-deficient transferrin (CDT) for the diagnosis of alcohol misuse. This phenomenon has only been observed when using the CDTect assay (Pharmacia & Upjohn, Uppsala, Sweden). In this study, we evaluated CDT in female PBC patients (n = 14) by a new CDT procedure, the %CDT turbidimetric immunoassay (TIA, Axis Biochemicals, Oslo, Norway) using the isoelectric focusing/immunoblotting/laser densitometry (IEF/IB/LD, Specialty Laboratories, Santa Monica, CA, USA) procedure as the gold standard. One of the PBC patients tested CDT+ by IEF/IB/LD (cut-off >9 densitometry units, DU) and %CDT TIA (cut off >6%); one patient tested at the cut-off point of the IEF/IB/LD and another one tested at the cut-off point of the %CDT TIA. Thus, unlike CDTect, the %CDT TIA is a procedure that produces few false positives in PBC.
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